there is a post MR816 "Audio Hardware Setup" in Cubase disappears - Legacy Steinberg Hardware - Steinberg Forums began in 2014 on this. Have you followed the instructions on this page: https://www.steinberg.net/en/support/knowledgebase_new/show_details/kb_show/mr816-resolving-audio-performance-issues.html I use a Startech 2 Port PCI Express FireWire Card (PEX1394A2) which has a Via - VT6315N chipset, on this page: https://www.steinberg.de/en/support/knowledgebase_new/show_details/kb_show/mr816-firewire-compatibility/kb_back/2025.html?tx_p77sbknowledgebase_pi1 another version is recommended, so I have searched for an alternative; some are very cheap and others very expensive. So may we all share which cards or onboard chipsets we are using?
Apologies, to those concerned, I should have said that I was following the path set by the Windows suggestions on the Steinberg pages and the previous Forum inputs, regarding this problem, which seemed to be solved, once and now is, possibly, more annoying and unpredictable than before!