Resolved !!
Wow guys I feel kinda stupid, but since it’s an honnest mistake i’m gonna put it here in case it happens to someone else.
When I was mixing I was switching to “Generic low latency asio driver” in “Studio setup” and was using my open headphones.
And for some reson this time when I switched back to “Focusrite USB asio” it did not routed properly the inputs in “audio connection” towards my audio interface. Once I changed it manually it worked fine.